Journal article

DVOŘÁK Pavel, KURUMBANG Nagendra, BENDL Jaroslav, BREZOVSKÝ Jan, PROKOP Zbyněk and DAMBORSKÝ Jiří. Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization. ChemBioChem. 2014, vol. 15, no. 13, pp. 1891-1895. ISSN 1439-4227. Available from:
Publication language:english
Original title:Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization
Title (cs):Maximalizace efektivity multi-enzymového procesu pomocí optimalizace stechiometrie
Journal:ChemBioChem, Vol. 15, No. 13, DE
biocatalysis, biotransformation, kinetic modelling
Multi-enzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of biocatalysts’ stoichiometry. Here we present a workflow for maximizing the efficiency of a three-enzyme system catalysing a five-step chemical conversion. Kinetic models of pathways featuring either wild-type or engineered enzymes were built and the enzyme stoichiometry of each pathway was optimized. Mathematical modelling and one-pot multi-enzyme laboratory experiments provided detailed insights into pathway dynamics, enabled the selection of suitable engineered enzyme and afforded high efficiency while minimizing biocatalyst loadings. The optimizing of stoichiometry in a pathway with engineered enzyme reduced the total biocatalyst load by an impressive 56 %. Our new workflow represents a broadly applicable strategy for optimizing multi-enzyme processes.
   author = {Pavel Dvo{\v{r}}{\'{a}}k and Nagendra Kurumbang
	and Jaroslav Bendl and Jan Brezovsk{\'{y}} and
	Zbyn{\v{e}}k Prokop and Ji{\v{r}}{\'{i}}
   title = {Maximizing the Efficiency of Multi-enzyme Process
	by Stoichiometry Optimization},
   pages = {1891--1895},
   journal = {ChemBioChem},
   volume = {15},
   number = {13},
   year = {2014},
   ISSN = {1439-4227},
   doi = {10.1002/cbic.201402265},
   language = {english},
   url = {}

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